This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation.One of the proteins is overexpressed in E.coli as HA-tagged recombinant protein and cell-free extracts Knife Rack are immunoprecipitated in HA-affinity resin.Cell extracts are treated with nuclease to degrade DNA and RNA, which rules o
Electrocardiograms (ECGs) provide important information for diagnosing cardiovascular diseases.In clinical practice, the conventional Ag/AgCl electrode is generally used; however, it is not suitable Art for long-term ECG measurement because of the risk of allergic reactions on the skin and the dying issue of electrolytic gels.In previous studies, s
Most sharks, rays and chimaeras (chondrichthyans) taken in commercial fisheries are discarded (i.e.returned to the ocean either dead or alive).Quantifying the post-capture Dual Oven Range survival (PCS) of discarded species is therefore essential for the improved management and conservation of this group.For all chondrichthyans taken in the main sh
Abstract In this paper, we propose the quantum semi-supervised generative adversarial network (qSGAN).The system is composed of a quantum generator and a classical discriminator/classifier (D/C).The goal is to train both Oven Side Support Rack the generator and the D/C, so that the latter may get a high classification accuracy for a given dataset.H